To Od Tosho - Naszego Strażnika Lymenetu.
Wstyd żeby caly Lymenet czył Polskie prace a my nic nie wiemy -
zobaczcie co oni znależli w tych kleszczach. wrrrr
1: Ann Agric Environ Med. 2004;11(2):319-22.
Studies on the occurrence of Gram-negative bacteria in ticks: Ixodes
ricinus as a potential vector of Pasteurella.
Stojek NM, Dutkiewicz J.
Department of Occupational Biohazards, Institute of Agricultural
Medicine, Jaczewskiego 2, 20-090 Lublin, Poland.
A total of 372 Ixodes ricinus ticks (101 females, 122 males, and 149
nymphs) collected by flagging in 6 mixed woodlands of eastern Poland
were examined by culture for the presence of internal Gram-negative
bacteria other than Borrelia burgdorferi.
Adult ticks were examined in pools of 2 specimens each and nymphs
were examined in pools of 3-5 specimens each. Ticks were disinfected
in 70 % ethanol and homogenized in 0.85% NaCl. The diluted
homogenate was inoculated onto 3 kinds of agar media: buffered
charcoal yeast extract (BCYE-alpha) for isolation of fastidious Gram-
negative bacteria, eosin methylene blue agar (EMB) for isolation of
enterobacteria, and tryptic soya agar for isolation of all other non-
fastidious Gram-negative bacteria.
The Gram-negative isolates were identified with the API Systems 20E
and NE microtests.
A total of
9 species of Gram-negative bacteria were identified, of which the
commonest were strains determined as Pasteurella pneumotropica/
haemolytica, which were isolated on BCYE-alpha agar from ticks
collected in all 6 examined woodlands.
The total number of these strains (49) exceeded the total number of
all other strains of Gram-negative bacteria recovered from ticks
Of the total number of examined ticks, the minimum infection rate
with Pasteurella pneumotropica/haemolytica was highest in females
(18.8%), and slightly lower in males (12.3%) and nymphs (10%).
the following species of Gram-negative bacteria were isolated from
Serratia plymuthica on EMB agar and
Pseudomonas aeruginosa, and
on tryptic soya agar. Minimal infection rates with these bacteria
were low, ranging from 0.7-5.9%. Of the isolated bacteria,
Chromobacterium violaceum, Pasteurella pneumotropica/haemolytica,
Pseudomonas aeruginosa, and Serratia marcescens are potentially
pathogenic for man and/or animals. In particular, the common
occurrence of Pasteurella pneumotropica/haemolytica in Ixodes
ricinus ticks poses a potential risk of pasteurellosis for humans
and animals exposed to tick bites.
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