Medical Diagnostic Laboratories !!!

[tosho]

Robią badania metodą Real-Time PCR

Ich oferta jest naprawdę imponująca!

www.mdlab.com/html/testing/available_tests.html#bacteriology
Zdaje się, że w przypadku niektórych bakterii są w stanie oznaczyć dodatkowe
parametry jak na przykład oporność bakterii na antybiotyki.

[klematis7]

jestem pod wrazeniem!!!
samych bartonelli badaja 5 gatunkow, mycoplasm tez 5.
Czy my dozyjemy czasow, ze tez bedziemy miec taki lab?

[tosho]

Przeczytajcie to. Na temat mykoplazm.
Technologia jest naprawdę niesamowita.



Another aspect of our lab work is the pyro-sequencing procedure. In this we are
a pioneering laboratory in the USA. It’s a techniquie in which you can amplify a
specific region within a bacterium or a virus (e.g. if this was an antibiotic
resistance region) eg Mycoplasma and give the Levofloxacin to a patient. - This
patient’s infection resists Levofloxacin, how can we find out this resistance,
but we know Levofloxacin is active, so take this specific region (that
Levofloxacin binds to) sequence it at where the gyrase binds and can find out
within 30 minutes the exact location where we have a mutation in this
Mycoplasma. We can find isolates that are resistant to antibiotics.
You can find this within half an hour. Can use the same technique to find
different genotypes within the ticks. We can tell them all apart.

This is again, same technique, OspC Pyrosequencing identification of Borrelial
species. (Re: the Borrelial species (burgdorferi, afzelii and garinii).

To distinguish between Bb and the European strains. European strains Afzelii and
garinii. Why is it important to differentiate? Both these strains are more
virulent and more associated with neuroborreliosis than Borrelia burgdorferi,
wheras B. burgdorferi (US strain) is more associated with arthritis. It’s
important to know the variations.

Way we can see that is if I align spot in - red, blue, green is where they
change, you can find regions where they look alike and we can amplify them and
amplify the regions where they don’t look alike. And that is how we tell the
difference between one and another. . Can differentiate between the species.

So, Uses of Pyrosequencing are: genus and species identification, secondly to
find point mutations/deletions/insertions and lastly to find antibiotic
resistance. Thank you.

www.lymediseaseaction.org.uk/conference/mordechai_2003.htm

[tosho]

Laboratorium to znajduje się w stanie New Jersey - może da się wysłać krew od
nas z Polski?

[tosho]

kolejny ciekawy lab
www.clongen.com/