artur737
16.01.07, 06:50
Wymyslono nowa bardzo skuteczna metode wykrywania kretkow w bioptatach tkankowych.
Jest to metoda immunochemiczna w ktorej probka jest automatycznie badana wedrujacym obiektywem. Autorzy okreslaja swoja metode jak znacznie skuteczniejsza od PCR-u i tania.
Ta metoda mozna np potwierdzic watpliwy rumien lub zdiagnozowac rumien wedrujacy w tych przypadkach gdy sam wyglad jest niejednoznaczny.
Focus floating microscopy: "gold standard" for cutaneous borreliosis?
Am J Clin Pathol. 2007 Feb;127(2):1-10.
Eisendle K, Grabner T, Zelger B.
Department of Dermatology and Venerology, Innsbruck Medical University, Innsbruck, Austria.
Borrelia burgdorferi is difficult to detect in routine biopsy material from patients with skin lesions of borreliosis.
In this study, a new immunohistochemical method, focus floating microscopy (FFM), was developed to detect B burgdorferi in tissue sections and was compared with polymerase chain reaction (PCR).
By using standard histologic equipment, tissue sections stained with a polyclonal B burgdorferi antibody were simultaneously scanned through 2 planes: horizontally in serpentines and vertically by focusing through the thickness of the section.
Borrelia were detected in 47 of 71 ticks, 34 of 66 tick bites, 30 of 32 erythema chronicum migrans cases, 41 of 43 borrelial lymphocytomas, and 50 of 51 acrodermatitis chronica atrophicans cases. FFM proved to be more sensitive than PCR (96.0% vs 45.2%) and nearly equally specific (99.4% vs 100%). All 169 control cases, except 1 false-positive case of secondary syphilis, were negative with FFM.
FFM is an easy, quick, and inexpensive method to reliably detect Borrelia in cutaneous tissue sections.
