artur737
14.11.08, 08:00
Szybki i niedrogi w Clongen Laboratories
This is Mycoplasma species detection in cell culture using Lonza's MycoAlert
System. The assay is a bioluminescent assays based on unique metabolic
processes that Mycoplasma species use to derive energy from their host cells.
The MycoAlert Kit specifically detects enzymes involved in Mycoplasma
metabolism that are absent in their host cells. By using substrates for the
Mycoplasma-specific enzymes, it is possible to drive the formation of ATP from
ADP.
The resultant ATP is then conveniently detected using the bioluminescent
reaction catalyzed by the Firefly Luciferase enzyme. Any increase in light
output from this reaction will indicate the presence of Mycoplasma.
The MycoAlert Kit has been extensively tested against all Mycoplasma and
Acholeplasma species commonly contaminating cells grown in culture.
Additionally, the assay has been compared to established methodologies such as
Mycoplasma culture, Hoechst staining and PCR. It was demonstrated that the
MycoAlert Kit exhibits equivalent or superior sensitivity to these methods and
provides more consistent, reliable results. By combining speed, ease of use
and sensitivity, the MycoAlert Kit provides the first effective tool to enable
cell biologists to tackle one of their most troublesome problems.
